Nephroprotective Effect of D-Ribose-L-Cysteine (DRLC) On Renal Oxidative Stress and Proinflammatory Markers in Lead Acetate-Treated Mice
DOI:
https://doi.org/10.71148/tjoc/v1i1.4Keywords:
Oxidative stress, Lead toxicity, Kidneys, Inflammation, D-Ribose-L-Cysteine, AntioxidantsAbstract
Lead exposure is known to cause damage to several body organs, including the kidney. In this study, the protective effect of D-Ribose-L-Cysteine (DRLC) on renal oxidative stress and proinflammatory markers in lead acetate-treated mice was investigated. Thirty-six (36) male Swiss albino mice were equally grouped into; 1 (Normal control); 2 (Negative control administered 100mg/kg of lead acetate only); 3 (Mice co-administered 100mg/kg of lead acetate and 50mg/kg of DLRC daily); 4 (Mice co-administered 100mg/kg of lead acetate and 100mg/kg of DRLC daily). The mice were treated, and sacrificed after 21 days. The kidney was excised and used to prepare homogenates for biochemical assay of glutathione (GSH), Catalase, Malondialdehyde (MDA), Myeloperoxidase (MPO) and Total protein. Results obtained revealed that the level of the oxidative stress marker, MDA, was significantly (p<0.05) elevated in the negative control group. However, coadministration of lead with DRLC led to significantly (p<0.05) reduced MDA levels when compared to the negative control group. The antioxidants (GSH levels and Catalase activity) were significantly (p<0.05) reduced following lead exposure, but coadministration with DRLC significantly (p<0.05) improved antioxidant defense in kidney of the mice. Also, exposure to lead increased inflammation in the mice as evident by significantly (p<0.05) increased MPO activity and Total protein levels, but coadministration with DRLC significantly (p<0.05) decreased MPO and total protein in comparison to the negative control group. This study, therefore, suggests that DRLC possesses significant nephroprotective potential in lead-exposed mice by mitigating the inflammation and oxidative stress induced by lead.
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