Changes in Pancreatic Antioxidant Biomarkers Activities Following the Ingestion of Abelmoschus Esculentum in Streptozotocin-Induced Diabetic Male Wistar Rats

Nduka Richard Ossai; Hope Idorenyi Ben; Chisom Treasure Obioma; Anthony Emeka Ojieh

doi:10.71148/tjoc/v1i1.6

Authors

Nduka Richard Ossai Department of Human Physiology, Delta State University, Abraka, Nigeria. Author
Hope Idorenyi Ben Department of Human Physiology, Delta State University, Abraka, Nigeria. Author
Chisom Treasure Obioma Department of Human Physiology, Delta State University, Abraka, Nigeria. Author
Anthony Emeka Ojieh Department of Human Physiology, Delta State University, Abraka, Nigeria. Author

DOI:

https://doi.org/10.71148/tjoc/v1i1.6

Keywords:

Glutathione reductase, Malondialdehyde, Nitric Oxide, Xanthine oxidoreductase.

Abstract

Type 2 Diabetes Mellitus (T2DM) is a metabolic condition involving high blood sugar and increased oxidative stress, with the pancreas being a primary target of the disease. This study aimed to investigate the significant changes in pancreatic antioxidant activities in diabetic rats treated with ethanol extract of Abelmonschus esculentum. Forty adult male Wistar rats were randomly divided into non-diabetic and diabetic units, each having four groups. Diabetes was induced in rats by a single intraperitoneal injection of streptozotocin (STZ) (600mg/kg). While Group 1 of both units was not treated, Group 2 received A. esculentum ethanol (300mg/kg) extract, Group 3 received N-acetyl Cysteine (NAC) (300mg/kg) and Group 4 received A. esculentum + NAC (300mg/kg). After 21 days of treatment, rats were euthanized and the pancreas were excised for biochemical analysis. Generated data were analyzed using GraphPad prism version 8.0, and results were expressed as Standard Error of Mean (SEM). The mean variations between groups were compared using a two-way analysis of variance (ANOVA), followed by the Turkey post hoc test, with p-values of 0.05 considered statistically significant. Results obtained showed a significantly decreased body weight, blood glucose, Xanthine oxidoreductase (XOR) and Malondialdehyde (MDA) levels with significantly increased Glutathione reductase (GR) and Nitric Oxide (NO) levels in treated groups indicating a positive mechanism in the management of oxidative stress induced by STZ. These findings suggest that A. esculentum extract has antioxidant properties that can mitigate oxidative stress in the diabetic pancreas, indicating its potential therapeutic value in management of T2DM.

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