Extraction and FTIR Spectra of Chitin and Chitosan Produced from Periwinkle (Tympanotonos fuscatus) under Differential Decolorization Conditions

Iruoghene Onosakponome; Prosper C. Okwuenu; Emeka H. Oparaji; Abigial O. Isojie; Gabriel E. Odesa; Ejiro P. Awhin; Sabinus O.O. Eze; Ferdinand C. Chilaka

doi:10.71148/tjoc/v1i1.7

Authors

Iruoghene Onosakponome Department of Biochemistry and Molecular Biology, Dennis Osadebay University, Asaba, Nigeria Author
Prosper C. Okwuenu Department of Biochemistry and Molecular Biology, Dennis Osadebay University, Asaba, Nigeria Author
Emeka H. Oparaji Department of Biochemistry, State’s University of Medical and Applied Sciences, Igbo-Eno, Enugu State, Nigeria. Author
Abigial O. Isojie Department of Biochemistry and Molecular Biology, Dennis Osadebay University, Asaba, Nigeria Author
Gabriel E. Odesa Department of Geology, Dennis Osadebay University, Asaba, Nigeria Author
Ejiro P. Awhin Department of Medical Biochemistry, Delta State University, Abraka, Nigeria Author
Sabinus O.O. Eze Department of Biochemistry, University of Nigeria, Nsukka, Nigeria Author
Ferdinand C. Chilaka Department of Biochemistry, University of Nigeria, Nsukka, Nigeria Author

DOI:

https://doi.org/10.71148/tjoc/v1i1.7

Keywords:

Chitin, Chitosan, Decolorization, Extraction, FTIR, Periwinkle

Abstract

This study aimed to extract and analyze chitosan sourced from periwinkle (Tympanotonos fuscatus) under differential decolorization conditions. Chitin was extracted from the shells of periwinkle (Tympanotonos fuscatus). The pulverized shells underwent demineralization with 2M HCl, deproteination using 1M NaOH, and decolorization to extract chitin. For chitosan extraction, deacetylation was performed with 40 % NaOH. Five solvents: 0.1 % hypochlorite, 1 % acetone, and 1 % ethanol - were employed to eliminate residual pigments from the chitin. FTIR analysis was conducted to examine structural variations resulting from the different decolorization treatments. The chitin yield varied between 51.70 % and 70.9 %, while the chitosan yield ranged from 28.04 % to 48.11 %. The degree of deacetylation was found to be between 46.3 % and 63.5 %, with the combination of acetone and ethanol yielding the lowest degree of deacetylation at 46.3 %. The FTIR spectrum of chitin extracted from periwinkle shells exhibited absorbance bands at approximately 3503.7, 2357.5, 1658.7, 1446.2, 1086.5, 886.6, and 678.4 cm⁻¹. These findings confirm that chitin can be effectively produced from periwinkle shells

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